![]() Recently, they have been heavily promoting a deal where you can get four lines of unlimited data for a total of $100. ![]() They have about 5,000 retail stores around the country, which isn’t as many as some of the more prominent national brands.You can assure yourself of getting an affordable rate with Cricket if you sign up for multiple lines with their Group Save program.I will end up paying $200 a month not a penny more. Without any installment plans or other discounts. Then for lines up to 10 lines each gets a $5 autopay discount. Ultimately, the going rate for Sprint MAX is the following (without any free lines): I'm not sure what the $173.73 number is made up of, do you have free lines and an insider discount? Then there is a section on the bottom for Included taxes and fees, where it breaks all of them down, but they are included so they don't actually add anything to the bill. I get billed $277.51 a month ($200 for 4 lines, 1 free line, and $87.51 my 3 payment plans, minus my $10 credit that will eventually expire). I have installments for $87.51 (it's 3 phones). When I look at my bill on my service is $190 (which I was quoted $200 when I switched to the plan for 4 lines). ![]() Then I have 5 lines now with 1 being free. I've got a promo $10 on one of my lines at the moment for an old issue. Sprint Emergency Response Team (ERT): Twitter, Facebook, Website New Cell Site Request Sprint: Twitter, Facebook, Google+, YouTube Günther Ottendorfer (Former COO, Technology): Twitter, YouTube, Reddit Communications): Twitter, Reddit Hall of Fame Tracy Nolan (President, National Sales, Strategy & Operations): Twitter Scott Rice (SVP, Integration and Transformation): Twitter Joe Meyer (VP, Service Management): Twitter Ryan Sullivan (VP, Product Development): Twitter Scott Santi (VP, Network Engineering & Deployment): Twitter Adrienne Norton (Corp. Officer): Twitter Deeanne King (EVP, Chief HR Officer): Twitter Cody Sanford (EVP, CIO, Chief Product Officer): Twitter Matt Staneff (EVP, CMO): Twitter Dow Draper (EVP, Emerging Markets): Twitter Callie Field (EVP, Customer Care): Twitter Jon Freier (EVP, Consumer Markets): Twitter John Saw (EVP, Advanced & Emerging Technologies): Twitter John Legere (Former Board Member, Former CEO of T-Mobile): Twitter Marcelo Claure (Board Member, CEO of SoftBank, Former CEO of Sprint): Twitter, Facebook Braxton Carter (EVP, CFO): Twitter Neville Ray (President, Technology): Twitter Janice Kapner (EVP, Chief Comms. Mike Sievert (CEO, Board Member): Twitter J. LTE Bands/Technologies by Device Social Media Request one for free.Ĭalling Plus Setup/FAQs Device Network Information
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*Please note that the 20% discount offer can’t be combined with other discounts, such as the educational and multi-license discounts. Please have a look at our FAQs for the Discount Campaign and the Pre-Order Campaign. You can learn more about our accepted payment methods. Final prices may vary depending on your country of residence. If you’re logged in to your Ableton account, the webshop will show your discounted prices and upgrade options.If your free Live 12 upgrade isn’t showing in your account, or you have any further questions about the discount offer, please get in touch.If you purchased a Live 11 license after Octoyou should have received an email notification that a free upgrade to Live 12 has been added to your Ableton account.For purchases of Push 3 (standalone), the offer is valid only until November 30, 2023. Shape the finest details of your sound with. This offer is valid from Novemuntil the release of Live 12 in early 2024. Komplete Start now comes with the brand-new EQ plugin from iZotopes industry-leading mastering suite, Ozone 11.Within the offer period, purchases of Live 11 Intro, Standard and Suite, with or without Push, also include a free upgrade to the equivalent Live 12 edition – to be issued upon release.Between November 14 and November 30, 2023, a 20% discount is also available on Push 3 (standalone).The 20% discount offer is valid for all Live 11 licenses and upgrades (including upgrades from educational licenses*), Live 12 pre-orders, Packs and cross-grades to Max 8 purchased during the campaign.To learn more about using Live, select a topic from our Learn Live tutorials or access the Live Manual from Live Help Menu Read the Live Manual. Once Suite is installed you will be able to access Max for Live within Live's Browser. Offer Terms & Conditions: Save 20% on Live 11 now, upgrade to Live 12 for free Overview Max for Live is built into Live 10 and 11 Suite. Updated for Live 11.1: Classic frequency shifting, ring modulation and real-time monophonic pitch shifting. Updated for Live 11: Redux adds a wider range of sounds from vintage digital gear. ![]() Updated for Live 11: Phaser-Flanger has a new, lusher sound with increased frequency and modulation ranges. Generowanie akordów i melodii za pomocą Orb Composer Suite 2.Updated for Live 11: Chorus now has a wider range of sound-shaping capabilities. Interfejs Scarlett 18i20 i OctoPre Dynamic Hi-endowe słuchawki Sennheiser HD800S do masteringuĪrturia Emulator II V - dlaczego warto go mieć? Suite is for the producer who wants all that, plus all the. Ableton Live Standard is for the producer who’s serious and wants to cast off their limitations. 13 more audio effects than Intro, and 256 mono audio inputs and outputs (contrasting Intro’s 8). Klawiatura komputerowa Editors Keys do Ableton Live Ableton Live 10 Suite (check price here) is the one with all the bells and whistles. Porównanie mikrofonów Shure SM7B vs Shure MV7 Teenage Enginieering OP-1 wciąż warte swojej ceny?Ībleton Live 11 | Obsługa nowych funkcji i efektów Kable SSQ | Wymiana kabli w mojej pracowni They share common features, but Standard and Suite have additional features, instruments, effects, and Packs. Słuchawki otwarte czy zamknięte? DT700 PRO X vs DT900 PRO X Live comes in three editions: Intro, Standard and Suite. Przenośna kabina wokalowa | Aston Microphones Halo
And with the Wi-Fi connectivity to allow you to monitor, preheat, and more, all from the SmartThings app on your smart device or use a voice command while lounging in the living room with friends. Outfitted with innovative features like Smart Dial that not only simplifies oven settings in a single dial it also learns your cooking preferences for your oven while making family meals. You can show off your cooking abilities in style with this technologically advanced Samsung 30” Fingerprint Resistant Stainless Steel Slide In Electric Range (NE63T8911SS). Best Slide In Electric Ranges: Our Top Picks Best Overall: Samsung 30" Slide In Electric Range (NE63T8911SS) This particular range is built to sit in between cabinets and has a top brim along the sides of the cooktop that only slightly overlaps the counter to blend into your kitchen design for a more seamless appearance. Why Trust Us? What is a Slide in Electric Range?Ī slide-in electric range is one of the three installation types you can purchase, including freestanding and built-in. At a Glance:īest Overall: Samsung 30” Slide In Electric Rangeīest Value: GE 30” Slide In Electric Rangeīest Splurge: Café 30” Slide In Electric Rangeīest Double Oven Slide In Electric Range: LG 30” Slide In Electric Double Oven Rangeīest Convection Slide In Electric Range: KitchenAid® 30" Slide In Electric Convection Rangeīest Slide In Electric Range With Air Fry: Whirlpool® 30" Slide In Electric Range with 7-in-1 Air Fry Ovenīest Induction Slide in Electric Range Under $1500: Frigidaire Gallery® 30" Induction Range with Air Fryīest Slide in Electric Range with Downdraft: KitchenAid® 30" Slide In Electric Downdraft Range Look no further and take a gander below at eight of our best slide in electric ranges that offer a sleek, custom built-in look, precision temperature control, and plenty of cooking features. Do you want a freestanding electric range, a built-in gas range, or maybe even an induction slide-in range? There are a variety of options and combinations, but we are here to convince you to envision just one of the arrangements for your kitchen. We survey thousands of CR members each year about the reliability of their gas, electric, and pro-style ranges, and we tabulate scores based on those responses.Buying any appliance for your home can be overwhelming when there are so many brands, models, fuel types, styles, and configurations to consider. (Here’s how to clean an oven without a self-clean setting.) For those with a self-clean feature, we bake a mixture of eggs, tapioca, pie filling, and a few other tough-to-remove foods onto the walls of the oven, then use the self-clean cycle and give each model a good wipe-down to see how effective the self-clean feature is at loosening grime. We measure and score usable capacity from the lowest rack position. Many manufacturers inflate oven sizes by counting the space where you can’t cook, like the bottom of the oven. And the broilers heat evenly while still getting hot enough to sear. Cakes and cookies emerge uniformly baked from multiple racks. They have a low-heat burner that’s capable of maintaining a steady temperature when simmering liquids, so you don’t need to stir constantly to avoid scorching, say, tomato sauce or chocolate. The best ranges in our tests are quick to bring a large pot of water to a near-boil. That’s why we recommend ranges only if they meet the following criteria. But what’s most important is a range’s ability to perform some very basic tasks really, really well, day in and day out. Their prices range from just under $500 to more than $9,000.Īt any retailer, you’ll find dozens of ranges-if not more-to choose from, some with fancier features than others. We test models from mainstream brands such as Amana, Frigidaire, GE, LG, Maytag, Samsung, and Whirlpool, and from pro-style manufacturers including GE Monogram, KitchenAid, Thermador, Viking, and Wolf. CR members can also access our complete range ratings. If you’re unsure of where to start, our range buying guide will tell you more about the types and features. ![]() ![]() Here, we highlight the best ranges (aka stoves) we’ve tested in our labs. Learn more about these credits and keep them in mind when choosing your next range. Passed last year, the legislation provides state-level credits of up to $880 for the purchase of many new electric and induction ranges and cooktops, as well as up to $500 in additional credits if you’re converting from gas. You can also save money, thanks to the Inflation Reduction Act. “We have some in the mix for $1,000 that perform better than models costing $5,000.” ![]() ![]() “We often find that price is no indicator of performance,” says Tara Casaregola, who heads CR’s testing of ranges, cooktops, and wall ovens. ![]() Certain clues will require items that need a certain skill requirement in order to wear or wield. These requirements are not strictly required as they can all be obtained from other sources or from other players. Please note that it is possible to receive the same clue more than once. In addition, beginner clue scrolls will not give master clue scrolls as a reward. After completing 600 beginner clues, the player will unlock the Explore emote.īeginner clue scrolls are not required to be given to Watson in exchange for a master clue scroll. ![]() One is given as a reward by Veos after completing X Marks the Spot should players not have one already in their possession.Ĭompletion of beginner clue steps will give another clue or a reward casket (beginner), containing the final reward for the trail. īeginner clue scrolls are the easiest to obtain and complete, and offer low rewards in return. Unlike all other clue types, which are equally likely to have each number of steps, beginner clues have a 10% chance of completion at 1 step and a 45% chance of completion at 2 or 3 steps. Beginner clues can be between 1-3 steps long. Īs for all tiers of clue scrolls, only one beginner clue scroll can be held, kept in death's office or stored in a bank at one time.įor a guide on how to complete a clue scroll, see Treasure Trails Guide. However, accounts that are less than 10 hours old will not be able to receive any unique rewards. Clue scrolls are the start of a Treasure Trail - a treasure hunt that sends players hunting all around Gielinor following a trail of clues that lead to rare and unique treasures. They can also be received from baby implings, young implings, clue bottles, clue geodes, and clue nests. ![]() A clue scroll (beginner) is a random drop from a large variety of lower-levelled monsters in free-to-play. It can also convert video files to audio MP3 and AAC formats. It can download songs or playlists from online platforms such as YouTube, Facebook, Dailymotion, Vimeo, Vevo, SoundCloud, and many more. The PRO version is priced at 19.95 and offers advanced options and. 5KPlayer is a free music player Mac solution that doubles as a video player and media streamer. The free version offers basic media-playing functionality. It is the free version of the more advanced Elmedia Player for Mac Pro, which features more advanced tools such as the ability to download online videos and movies, full-screen mode and a snapshot tool. Elmedia Player can be acquired as a Free version or a PRO version. Subtitle support and options for picture-in-picture as well as streaming to your device, makes for an exceptional media player. Elmedia Player for Mac Free is a multifunctional media player for Mac OS X, which can play a great variety of formats. The software lets users adjust brightness, saturation, contrast, hue, gamma, and sharpness and even reduce the noise on certain videos. It is the Pro version of Elmedia Player for Mac Free and features more advanced tools such as the ability to download online videos and movies, full-screen mode and a snapshot tool. Elmedia Player for Mac Pro is a multifunctional media player for Mac OS X, which can play a great variety of formats. Installation is very simple whether you go directly to the Elmedia website or download through the Mac store.Īs well as reliably playing many video types, you can choose to view them in your own way. A different way to view and manage media files. This means that there may actually be no need to convert AVI to MP4 on Mac.Įlmedia runs natively on all Mac computers, including the recent releases with Apple Silicon, including the M1 and M2 series. The software is a sophisticated media player that gives you numerous playback and customization options, as well as supporting an incredible amount of video and audio formats. ![]() Before taking the decision to turn AVI into MP4 files, consider whether it might just be a whole lot easier to play AVI, MP4, and a variety of other videos directly on your Mac with no need for conversion at all.Įlmedia Player can fix many of the problems Mac users have with video files. ![]() There are a few great FREE alternatives to Camtasia Studio to bring out the teacher in you. Now, what if you release occasional instructional content and you are planning on splurging $249 on this software? Well, we’ve got you covered. Camtasia alternatives for Mac & Windows to record screen If you want to see what kind of videos you can create using this amazing video tool for bloggers, check out ShoutMeLoud’s official YouTube channel. This software would definitely not disappoint you. You could also download the free trial and see for yourself if you decide to make the plunge. It is worth a one-time investment for such users. Camtasia es una aplicación muy útil que se puede utilizar para crear el video de las actividades en pantalla desde cualquier aplicación. How to Download and Install Camtasia on Your PC for Free Click on the Download button on the sidebar to go directly to the official Camtasia download page. The price for the software is justifiable if the user is a regular maker of such content. Camtasia 2023 Full es la mejor aplicación para grabar pantalla y editar videos todo en uno (TEU-AIO), esta aplicación facilita la grabación y creación de videos de aspecto profesionales. That’s a lot for someone who releases occasional instructional videos or content. Given all its benefits, its latest version is priced at a steep $249! Camtasia Studio allows you to create powerful screen recorded videos with additional content of your own, ready-to-use themes, animated backgrounds, graphics, callouts, and clickable links in videos that make it the best software to engage your audience. The fact that it is available for both Mac and Windows along with its ton of unique features, it is a viable option for those who make a good deal of instructional matter and video guides for their YouTube channel.Ĭamtasia Studio offers out of the box features that would make you forget the need for any other software for making instructional content. ![]() Even Harsh uses the same software for his YouTube channel, and it’s also listed in ShoutMeLoud’s blogging toolkit. ![]() The electron configuration of an element with an atomic number greater than 18 cannot be properly determined according to the Bohr atomic model. Electrons can be arranged correctly through orbits from elements 1 to 18. Therefore, the order of the number of electrons in each shell of the radon(Rn) atom is 2, 8, 18, 32, 18, 8. Therefore, a radon atom will have two electrons in the first shell, eight in the 2nd orbit, eighteen electrons in the 3rd shell, and thirty-two in the 4th shell.Īccording to Bohr’s formula, the fifth shell will have twenty-six electrons but the fifth shell of radon will have eighteen electrons and the remaining eight electrons will be in the sixth shell. ![]() That is, the number of electrons in radon is eighty-six. The atomic number is the number of electrons in that element. Therefore, the maximum electron holding capacity in the first shell is two, the second shell is eight and the 3rd shell can have a maximum of eighteen electrons. The maximum electron holding capacity in N orbit is 2n 2 = 2 × 4 2 = 32. ![]() The maximum electron holding capacity in M orbit is 2n 2 = 2 × 3 2 = 18. The maximum electron holding capacity in L orbit is 2n 2 = 2 × 2 2 = 8. The maximum electron holding capacity in K orbit is 2n 2 = 2 × 1 2 = 2. The electron holding capacity of each orbit is 2n 2. K is the name of the first orbit, L is the second, M is the third, and N is the name of the fourth orbit. These circular paths are called orbit(shell). The electrons of the atom revolve around the nucleus in a certain circular path. The complete idea of the orbit is given there. Scientist Niels Bohr was the first to give an idea of the atom’s orbit. Radon atom electron configuration through orbit For example Aufbau principle, Hund’s principle, and Pauli’s exclusion principle. Coenzyme B12 provides the C-Co bond, which is part of the reactions.Radon atom electron configuration (Bohr model)Įlectron configuration through orbitals follows different principles. The absence or low presence of cobalt can cause anemia.Ĭobalamin proteins use the corrin ring to hold the cobalt unit. It makes up cobalamin, also called vitamin B12. Biological function of cobaltĬobalt is needed in all animals and in humans as well. In the absence of nuclear war, the risk arises from improper maintenance or handling of radiotherapy units. This is why certain nuclear weapons called dirty bombs are created. If a nuclear confrontation were to occur, the emission of neutrons could convert iron into Co-60, greatly increasing the consequences of radioactive contamination.Ingestion of Co-60 can cause a certain amount to accumulate in the tissues, which is eliminated slowly. Co-60 is radioactive, exposure to its radiation can cause cancer.Generally, cobalt compounds should be handled with care as the metal has mild toxicity. Finely separated metallic cobalt powder is flammable.For this reason, these fonts have ceased to be popular in the West. The Cobalt-60 source is composed of a useful life of about 5 years, but when this duration is exceeded, the isotope is still quite radioactive. Metal normally develops a fairly fine dust which makes radiation shielding difficult. The source used has a radius of 2 cm and produces the presence of penumbral parts that diffuse the radiation that surrounds its direction. it is therefore difficult to be able to encapsulate it to avoid radioactive contamination.Ĭobalt-60 generates two gamma rays that have energies of 1.33 MeV and 1.17 Mev. Isotopes lighter than the stable Co-59 are primarily decayed by electron capture creating iron isotopes, while those heavier than the stable isotope are decayed by beta emission giving rise to nickel isotopes.Ĭobalt-60 is used in radiotherapy instead of radium because of its economic price and also because radium decays into radon and it is a radioactive element in the form of a gas. ![]() The atomic mass of isotopes of this element is between 50 amu and 73 amu. Cobalt also has four meta-states, each of which has half-lives of less than 15 minutes. The other radioactive isotopes have half-lives of less than 18 hours and almost all of them less than one second. The most stable are Co-56, Co-57 and Co-60 whose half-lives are 70.86 days, 271.79 days and 5.2714 years respectively. The boiling point of this element is 2927.85 degrees Celsius or 3200 Kelvin and the melting point is 1495.85 degrees Celsius or 1768 Kelvin. This chemical element has a gray tint, is metallic and belongs to the group of transition metals, its atomic number is 27 and it is represented by the symbol Co. The covalent radius of cobalt is 126 pm, the Bohr radius or atomic radius is 152 pm, while the average radius is 135 pm.Ĭobalt is solid in its natural form, specifically a ferromagnetic solid. Liquid, shows up dental plaque during the scale removal or dental prophylaxis phase. The Newtron® SLIM B.LED fitted with its Blue LED ring and used in conjunction with the F.L.A.G.™ disclosing ![]() Or a dental file attached to the end of the ultrasonic handpiece. Mechanical vibrations are transmitted to a tip Which transforms the electrical signal into ultrasonic vibrations. The handpiece comprises a piezoelectric ceramic transducer, This isĬonnected to the medical device via a cord. It is designed for the treatment of prophylaxis, periodontics, endodontics and preservation and restoration dentistry.Īn electrical signal emitted by the medical device is supplied to the dental ultrasonic handpiece. This medical device is used in association with a dental utrasound handpiece to which an ultrasound instrument We recommend that you visit the website regularly to consult and/or to download the latest version of your Your device and its accessories prior to use. It is important for you to have read and understood the content of the user instructions relating to the use of The device user instructions can be consulted at the following address: Have a PDF file read software installed to read the instructions. The electronic user instructions are available in PDF format (Portable Document Format) and you will need to ![]() However, youĬan request a free printed copy of the user instructions within 7 days via our website, by telephone or in writing. The user instructions for your device are provided in electronic format and not in printed format. Method for consulting electronic user instructions General instructions relating to the complete range ofSATELEC® dental ultrasonic generators This document must be used in association with the following documents:Ĭleaning, disinfection and sterilization protocols for Wrenches SATELEC®Ĭleaning, disinfection and sterilization protocols for Tips SATELEC®Ĭleaning, disinfection and sterilization protocols for Handpieces SATELEC® L maintenance to be performed by the user. L monitoring and general maintenance of the medical device L preparation for cleaning and disinfection of the medical device This document contains the following information: L recommendations for the inspection of parts. L information concerning conditioning for sterilization L detailed manual and automatic protocols L preparation of parts for sterilization Please refer to the accessory cleaning, disinfection and sterilization protocols and the handpiece predisinfection, cleaning and sterilisation protocols for information about the following: L disposal and recycling of the medical device ![]() L the medical device usage interactions, contraindications and prohibitions L warnings concerning user and patient populations Please refer to the instructions relating to the comprehensive range of dental ultrasonic generators SATELEC® for information about the following: If you have received this medical device by mistake, please contact the supplier to arrange To benefit fully from the technology of your medical device, please read the documentation To ensure optimum safety for yourself and your patients, comfort in your daily practice and It comprises the chosen tool with which you will provide treatment within the context of your work. The medical device SATELEC® that you are about to install and use in your practice is a medical device designed for professional use. 3 Removal from packaging, installation, connectionsģ.4 Connecting the medical device to the water systemģ.5 Connecting the medical device to the electrical networkģ.6 Plugging the medical device to the electrical networkģ.8 Fix the medical device to a non-removable supportĤ.6 Connecting and disconnecting accessories during useĤ.11 Irrigation flow configuration buttonĤ.17 Activating ultrasounds using the pedalĥ.1 medical device cleaning and disinfectionĥ.2 Cleaning and disinfecting accessoriesĦ Monitoring and maintenance of the medical deviceĨ Technical specifications for the medical device While Clamps says "Snitch juice," the subtitles read "Hoffa Juice.".It is the first time that Clamps' real name is used.broadcast on 14 July, 2011, "The Silence of the Clamps" scored a 0.7 share among adults 18-49 and 1.406 million viewers, down about 150,000 viewers since " Law and Oracle", making it the second lowest rated episode of season 6 after " The Futurama Holiday Spectacular". This episode was subject to censorship by itself. " Neutopia" replaced the episode as the eighth broadcast season's first.ĭuring May and June 2011, Countdown to Futurama released three items of promotional material for the episode: reused concept art of the Crushinator from " The Series Has Landed" (accompanied by two classic Crushinator clips) on 22 May, part of the storyboard showing the Donbot's trial on 23 May, and a video clip featuring Clamps arguing with Zoidberg on 17 June. A video clip from the episode featuring Bender meeting Bella and witnessing the crime was hidden on Volume 5 as an Easter egg. Eric Rogers said on his Twitter page that the episode would be directed by Frank Marino, not Ray Claffey. Maurice LaMarche revealed in an update online that the episode would have a large focus on Clamps, indeed his great breakthrough episode. "The Silence of the Clamps" was originally titled " Bend on a Wire". With the Robot Mafia believing Bender to be dead, Bender is freed from witness protection. There they find Bender working as a waiter, revealing himself to still be under witness protection there, and realize that Billy was a different, innocent robot. The crew returns to Earth to mourn Bender's apparent death at Family Bros. However, Bella arrives and shoots "Billy" to death, accusing him of cheating on her since he is married to the Crushinator. ![]() This does not stop Clamps from attempting to kill "Billy," but he is stopped by Zoidberg, who uses his pincers to cut off his clamps. The Planet Express crew makes a delivery to the moon where they find a robot they believe to be Bender, who claims his name is Billy West and does not seem to remember any of them, his memory seemingly erased as part of the witness relocation program. However, he is unsuccessful and earns the resentment of Zoidberg, who fears that Clamps's masterful use of his clamp-like hands will overshadow the use of his own pincers, the only part of Zoidberg's job that makes him feel appreciated. Determined to hunt down Bender, the Donbot sends Robot Mafia member Clamps to apply for Planet Express under his real name, Francis, in order to befriend Fry and make him reveal Bender's location. Now targeted by the Robot Mafia for ratting out the Donbot and his affair with Bella, Bender is forced to go into witness relocation, leaving Planet Express to begin hiring for his replacement. However, he is recognized by Bella, while Calculon is threatened by the Robot Mafia into testifying in favor of the Donbot's innocence. ![]() He secretly makes out with the Donbot's daughter Bella when he witnesses the Robot Mafia viciously beating Calculon.īeing the only witness of the attack, but fearing repercussions from the Robot Mafia, Bender tries to disguise himself while testifying against the Donbot in open court. While making a delivery to the Donbot's mansion during a wedding, Bender sneaks onto the mansion grounds to take part in the festivities. ![]() It aired on 14 July, 2011, on Comedy Central.Īfter testifying against the Robot Mafia, Bender goes into hiding in the witness relocation program.Įditors are encouraged to update and expand the article. " The Silence of the Clamps" is the one hundred and second episode of Futurama, the fourteenth of the sixth production season and the fifth of the eighth broadcast season. Primetime Emmy Award for Outstanding Voice-Over Performance, Maurice LaMarche as Clamps, Donbot, Hyper-Chicken, Calculon, Hedonismbot, and Morbo, 2012 ( Won) ![]() Robot Stack tells Bender that the EBI is putting him in the witness relocation program. ![]() ![]() Antigen-specific memory B cells expressing surface IgG and IgG-secreting plasma cells have been exploited extensively as a source of mAbs. Moreover, this technique exploits the natural process of affinity, specificity, and stability profiles of mAbs. This technology avoids the inefficient hybridoma fusion step and retains the natural heavy and light chain pairing, thereby allowing a more thorough interrogation of the B cell population. More recently, a single B cell-based method has been developed that allows direct sampling of the immune repertoire from a single B-cell or the clonally expanded progeny of single cell ( 7). In addition, the natural cognate pairing information of antibodies is lost in display methods, which reduced the specific diversity of antibodies. Although these antibody generation technologies were widely adopted for mAbs screening, these methods were inefficient and required time-consuming operations. In the past few decades, several display techniques such as phage display ( 3), yeast surface display, ribosome display ( 4, 5), and mRNA display technologies ( 6) have been used for producing mAbs. The mouse hybridoma method described by Kohler and Milstein in 1975 was the first and most widely used approach for obtaining mouse mAbs ( 2). Many methods exist for the generation and identification of mAbs for both research and therapeutic purposes. mAb therapeutics has revolutionized the approach to many serious human diseases with an increasing speed, and over 230 mAbs were evaluated in phase clinical studies in early 2017 ( 1). Monoclonal antibodies (mAbs) are essential tools in biochemistry, molecular biology, and medicine research. With advances in antibody function and repertoire analysis, rabbit mAbs will be widely used in therapeutic applications in the coming years. This review describes the basic characterization of rabbit antibody repertoire and summarizes methods of hybridoma technologies, phage display platform, and single B cell antibody technologies. During the past decade, many techniques have been developed for isolating rabbit mAbs, including single B cell antibody technologies. The rabbit monoclonal antibodies (mAbs) have advantages in pharmaceuticals and diagnostics with high affinity and specificity. ![]() 3State Key Laboratory of Organ Failure Research, National Clinical Research Center for Kidney Disease, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, China.2College of Life Science, Xinyang Normal University, Xinyang, China.1Institute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China.Huijuan Liu 1,2 Qian Guan 3 Lei Wang 1,2 Hongyu Yuan 1,2 |
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