![]() ![]() Antigen-specific memory B cells expressing surface IgG and IgG-secreting plasma cells have been exploited extensively as a source of mAbs. Moreover, this technique exploits the natural process of affinity, specificity, and stability profiles of mAbs. This technology avoids the inefficient hybridoma fusion step and retains the natural heavy and light chain pairing, thereby allowing a more thorough interrogation of the B cell population. More recently, a single B cell-based method has been developed that allows direct sampling of the immune repertoire from a single B-cell or the clonally expanded progeny of single cell ( 7). In addition, the natural cognate pairing information of antibodies is lost in display methods, which reduced the specific diversity of antibodies. Although these antibody generation technologies were widely adopted for mAbs screening, these methods were inefficient and required time-consuming operations. In the past few decades, several display techniques such as phage display ( 3), yeast surface display, ribosome display ( 4, 5), and mRNA display technologies ( 6) have been used for producing mAbs. The mouse hybridoma method described by Kohler and Milstein in 1975 was the first and most widely used approach for obtaining mouse mAbs ( 2). Many methods exist for the generation and identification of mAbs for both research and therapeutic purposes. mAb therapeutics has revolutionized the approach to many serious human diseases with an increasing speed, and over 230 mAbs were evaluated in phase clinical studies in early 2017 ( 1). Monoclonal antibodies (mAbs) are essential tools in biochemistry, molecular biology, and medicine research. With advances in antibody function and repertoire analysis, rabbit mAbs will be widely used in therapeutic applications in the coming years. This review describes the basic characterization of rabbit antibody repertoire and summarizes methods of hybridoma technologies, phage display platform, and single B cell antibody technologies. During the past decade, many techniques have been developed for isolating rabbit mAbs, including single B cell antibody technologies. The rabbit monoclonal antibodies (mAbs) have advantages in pharmaceuticals and diagnostics with high affinity and specificity. ![]() 3State Key Laboratory of Organ Failure Research, National Clinical Research Center for Kidney Disease, Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou, China.2College of Life Science, Xinyang Normal University, Xinyang, China.1Institute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, China.Huijuan Liu 1,2 Qian Guan 3 Lei Wang 1,2 Hongyu Yuan 1,2
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